digital PCR (dPCR) sample analysis using the QIAcuity system
At BioXpedia we offer digital PCR (dPCR) sample analysis using the QIAcuity system.
dPCR enables absolute quantification of target nucleic acid molecules (DNA, RNA and oligos). For quantification we use the QIAcuity system from QIAgen. It utilizes a Nanoplate, where each well is divided into a large number of partitions (micro chambers of nl-size). One sample is added per well, and the molecules in each sample are distributed randomly into the partitions. In each of the partitions target end-point PCR amplification will take place, and amplification is detected by measuring fluorescence. Some partitions will contain either one or a few target molecules (positive count) while other partitions will be empty (negative count). Due to the random distribution in the partitions, Poisson statistics can be used to calculate target quantification without the use of a standard curve.
Types of Nanoplates
- 24-well plate with 26.000 partitions
- 24-well plate with 8.500 partitions
- 96-well plate with 8.500 partitions
The PCR reaction will use target specific primers and inter-chelating EvaGreen dye or fluorescent probes. 5 target detection channels are available for multiplexing.
Examples of fluorphores
- FAM, EvaGreen (Green)
- HEX, VIC, JOE (Yellow)
- TAMRA, ATTO 550 (Orange)
- ROX, Texas Red (Red)
- Cy5 (Crimson)
Contact BioXpedia for more info about our dPCR service