Meso Scale Discovery Immunoassays
Absolute and Multiplex Quantification of Proteins
At BioXpedia we offer protein quantification services using immunoassays from Meso Scale Discovery. In drug discovery and biomarker identification, absolute quantification of proteins can be relevant in order to determine the importance of observed differences in protein levels between patient groups. ELISA is a widely used assay for the quantification of cytokines and other proteins in many clinical samples, but ELISA is cumbersome for multiple analytes.
The Meso Scale Discovery (MSD) technology offers absolute quantitation of protein markers by using multiplex immunoassays based on electrochemiluminescence. The technology provides a higher sensitivity and broader dynamic range than are possible by using traditional ELISA. It is highly flexible and assays can be provided as single- or multiplex assays with up to 10-plex in each well, saving precious sample material.
Multiplex protein quantification
MSD immunoassays are based on a proprietary technology combining electrochemiluminescence and multiarray technology for detection of multiple proteins in a single sample. The majority of assays are sandwich-based and consist of a Multi-Spot microplate, where each spot is coated with unique capture antibodies. After incubation with the sample, binding of analytes are detected via SULFO-TAG conjugated antibodies. When electricity is applied to the plate, light is emitted from the SULFO-TAG, and intensity measured for quantification of analytes in the sample.
The Multi-Spot microplates are available in a 96- well format and allows multiplexing of up to 10 assays in each well, making MSD a rapid technology for multiplex protein quantification.
More Than 500 Assays Available
Available MSD assays cover a wide range of biological applications, with more than 500 assays provided in panels, custom panels, or reagent kits to build assays.
The biomarker panels are focused on measuring proteins associated to e.g. inflammation, oncology, neurodegeneration, immunology, intracellular signaling, cardiovascular disease and metabolism. Assays are available for different species and are compatible with an array of complex sample types e.g. serum, plasma, supernatants, cell lysates, CSF, urine, blood spots, tears, synovial fluid, tissue extracts etc.
The Different Types of Immunoassays are:
- V-PLEX (Validated assays)
- U-PLEX (Custom multiplex of assays)
- R-PLEX (Custom assay design)
- S-PLEX (Extra sensitivity assays)
V-PLEX assays are designed to maximize reproducible performance, consistency and reliability in results and confidence in data. The assays and panels are fully validated in multiple matrices according to fit-for-purpose principles. The validated assays come in both separate assays or multiplex variants and are designed to ensure optimal and consistent performance. Lot-to-lot consistency is guaranteed to support long-term studies.
The U-PLEX assays offer full flexibility to custom design your own singleplex or multiplex assays. In the U-PLEX assays biotinylated capture antibodies are coupled to U-PLEX linkers, which then bind to specific spots on the plate. Besides antibodies, the linkers can be used with other biotinylated capture-reagents such as proteins, peptides, or nucleic acids, allowing you to study the desired proteins unique for your project.
The R-PLEX antibody sets can also be used for development of multiplex or single analyte immunoassays. Each R-PLEX antibody set contains a biotinylated capture antibody, electrochemiluminescence labelled detection antibody and a recombinant protein for standard.
Finally, the S-PLEX assays can be used for detection of very low concentrations of protein. An optimized detection antibody increases the sensitivity of the assays, with a detection limit down to the low fg/ml.