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Fluidigm Biomark HD and Juno Systems

fluidigm

High-throughput analysis in nanoscale reactions

The Fluidigm systems for genomics and gene expression enable high-throughput and sample-efficient analyses in low volume reactions.

Fluidigm has developed chips or plates with integrated fluidic circuits (IFCs), also referred to as Dynamic Arrays – pictured below.. The technology allows rapid and reproducible multistep sample preparation and analysis of up to hundreds of genetic markers across thousands of samples in just hours, rather than days or weeks.

The principle of the technology is based on the primer and probe pairs being loaded on one side of the IFC array frame, and the samples are loaded on the other. Once in the wells, the components are pressurized into the chip using an IFC controller. The components are then systematically combined, so that in each chamber of the chip one sample is mixed with one assay and the reaction takes place here.

At BioXpedia we combine the Fluidigm instruments, Biomark HD and Juno, which enables maximal efficiency and workflow. We employ the Juno instrument as controller, preparing the IFC chips, which is then run on the Biomark HD in either real-time or endpoint read mode.

Integrated fluidic circuit for efficient and reproducible analysis

The IFC chip comes in different variants allowing flexibility regarding number of samples and assays.

Chip variants:

  • 12.12 – 12 samples x 12 assays (144 reactions)
  • 48.48 – 48 samples x 48 assays (2,304 reactions)
  • 96.96 – 96 samples x 96 assays (9,216 reactions)
  • 192.24 – 192 samples x 24 assays (4,608 reactions)
  • 24.192 – 24 samples x 192 assays (4,608 reactions)

The Fluidigm qPCR system supports several genomics-based applications, such as gene expression analysis, both mRNA and miRNA, SNP-genotyping, digital PCR, mutant detection, and many more. In addition, we use the Fluidigm Biomark for quantification of protein in combination with Olink Protein screening.

Fluidigm instruments, Biomark HD and Juno

Fluidigm chip with integrated fluidic circuits (IFCs)

SNP Genotyping

At BioXpedia we provide SNP genotyping service, using the Fluidigm systems allowing high-throughput and maximum reproducibility.

Genotyping can be key to understanding disease or to predict treatment response of a specific drug. Several technologies, like Next Generation Sequencing (NGS), micro arrays or targeted SNP genotyping on the Fluidigm platform can be used in genotyping studies, depending on the aim of the study. Genotyping allow researchers to study genetic variations across the genome between different groups of patients. These variations can be both small differences, such as single nucleotide polymorphisms (SNPs) or larger structural DNA changes, e.g. loss of heterozygosity and copy number variation (CNV).

The Fluidigm system for SNP genotyping is a fast and reproducible method as multiple samples can be evaluated in a single run, and the workflow is almost entirely automated requiring minimal hands-on time.

Genotyping using Fluidigm requires only small amounts of DNA, making it ideal for analysis of precious samples, and the system is compatible with multiple types of assays, including:

TaqMan assays include allele-specific probes, while SNP Type and IDT rhAmp SNP assays are based on universal probes, and all assay types allow customized assay design.

Gene Expression (qPCR)

BioXpedia offers qPCR service using the Fluidigm system, which offers flexibility and high throughput.

Analysis of gene expression is the first level of exploration for any regulation at the molecular and cellular levels. Transcription of genes is a dynamic process, allowing cells to adapt rapidly to external, environmental or physiological changes.

Fluidigm allows rapid and reproducible results employing multiplex gene expression assays. Thus, a gene expression profile of selected genes ranging from 12 to 192 genes, can be determined in a single run, using very little RNA as input.

Analysis of miRNA expression have likewise gained attention, as miRNAs have been found to be important regulators of several processes in the human body. miRNAs are typically found and exert their mechanism within the cells, however circulating miRNAs and exosomal