Digital pcr

RNA/DNA quantification with superior accuracy, precision and reproducibility

Our Service

Digital PCR 

Digital PCR Technology

BioXpedia offers digital PCR (dPCR) which enables RNA/DNA quantification with superior accuracy, precision and reproducibility.

dPCR needs no standard curve or reference samples, and the technology excels in complex samples of low target abundancy and high wild-type background. 5 detection channels allow for multiplexed quantification.

Technologies:

Digital PCR using QIAcuity System  

Examples of applications are

  • Rare mutation detection (e.g. circulating tumor DNA)
  • Gene expression studies
  • Genotyping
  • Copy number variation (CNV) detection
  • Gene editing detection (CRISPR/Cas9)
  • Quantification of NGS libraries
  • Pathogen detection

Sample Types for dPCR

DNA and RNA extracted from all sources can be used as templates for dPCR. It is important with a high purity of the nucleic acids, as contaminants (such as proteins, salts, EDTA and phenol/chloroform) can interfere with the enzymatic reactions and fluorescence detection. Our lab can assist with high quality extraction from most biological samples.

  • Fresh or frozen tissue
  • FFPE tissues
  • Cultured cells
  • Whole blood
  • cfDNA from plasma or serum
  • Other – please contact us

 

Please contact us to learn more about our QIAcuity dPCR Service, and how we can assist you in your specific project.